Br J Pharmacol. Oct;(4) doi: /j x. Experimental autoimmune encephalomyelitis (EAE) as a model for multiple. La inducción de la encefalomielitis autoinmune experimental (EAE) mediante la glicoproteína de la mielina del oligodendrocito (MOG) en ratones C57BL/6. Keywords: multiple sclerosis, encephalomyelitis, autoimmune, experimental, Latin Estudios sobre la encefalomielitis autoinmune experimental (EAE) en.

Author: Tajas Gardahn
Country: Turkmenistan
Language: English (Spanish)
Genre: Spiritual
Published (Last): 5 July 2007
Pages: 184
PDF File Size: 14.35 Mb
ePub File Size: 9.86 Mb
ISBN: 148-7-79899-222-6
Downloads: 85761
Price: Free* [*Free Regsitration Required]
Uploader: Voodoozilkree

During some stages, clinical evaluation may not follow or mimic the behavior of molecular parameters, and body weight was therefore explored solely in a descriptive manner. One-milliliter plastic syringes were used for the immunizations, loaded directly from the glass syringe by means of the metal capillary. The evaluation by qPCR of the changes in cytokine profile encefalomiielitis after the induction of EAE, as well as the histological findings of the transmission electron microscopy study, confirmed that the EAE model had indeed been implemented.

Granulocyte macrophage colony-stimulating factor: It has been shown that blood leptin concentrations increase after the induction of EAE, correlating with the decrease in body weight that takes place during part of the autoimmune process [33]. Pathological elements manifest in about 7 to 14 days when EAE is actively induced but appear more quickly upon adoptive transfer of activated myelin-specific T cells.

In addition, EAE models have been essential for research on the modulation of immunoregulatory circuits, a field that has provided important results for our understanding of the pathogenesis of MS. The positive effect of hydrocortisone on the clinical symptoms of EAE-affected animals Figure 1A was corroborated with statistical comparisons Figure 1B.

Key clinical features Classic EAE in mice causes so-called flaccid paralysis characterized by decreased muscle tone that progresses from the tail upward along the body. Se centrifuga a 1. IL plays an important role in the development of experimentxl autoimmune encephalomyelitis. Experimentla, its contribution to esperimental endeavor is controversial: A particular mouse strain might resist EAE induced by one approach but succumb to the other—and figuring out why can reveal important molecular mechanisms, Becher adds.

EAE provides a means for investigating mechanisms of autoimmune-related CNS damage and demyelination.

Experimental Autoimmune Encephalomyelitis | Multiple Sclerosis Discovery Forum

Each inoculation delivered a total of ng of PT per animal. In order to detect motor irregularities while walking, the mice must be placed onto a flat surface. Procuring preclinical efficacy data is a fundamental step in the development of new drugs.


After the initial inflammatory attack subsides, the animals go into remission and later expetimental episodes that involve inflammation, demyelination, and axonal encefalomieltis Merrill, However, similar to MS, the disease symptoms reflect the anatomical location of the inflammatory encefalomiflitis, and may also include emotional lability, sensory loss, optic neuritis, difficulties with coordination and balance ataxiaand muscle weakness and spasms.

This procedure is not equally successful with every animal species, however —especially when using myelin oligodendrocyte glycoprotein MOG for this purpose— due to variations in genetic background and allele polymorphisms of class II histocompatibility complex molecules and T-cell receptors [9]. The difference between the observed body weight and that measured at day 0 of the study was determined daily, calculating group means to follow the behavior of this variable.

Experimental autoimmune encephalomyelitis (EAE) as a model for multiple sclerosis (MS).

The typical susceptible rodent will debut with clinical symptoms around two weeks after immunization and present with a relapsing-remitting disease. In addition, environmental factors can be determinant in the development of EAE. IL and Th17 Cells. The multiple sclerosis market.

Their findings upon a transfer of inflamed patient tissue to primates was published in the Journal of Experimental Medicine. Baker and his colleague Sandra Amor at VU University Encetalomielitis Center in the Netherlands laid out a set of guidelines that proposes minimally acceptable standards for publishing studies that use EAE as a preclinical model Baker and Amor, Experiments should be done in an actively encefalomielittis model and a passive transfer model.

Experimental autoimmune encephalomyelitis EAE. In monophasic or relapsing-remitting EAE, varying degrees of remyelination occur.

Centrifugar a xg durante 2 min a TA. Apprentice yourself to an experienced EAE researcher who can critique your scientific plan, Becher suggests: Transforming growth factor beta is dispensable for the molecular orchestration of Th17 cell differentiation. The maximum clinical score was 3.

Tips The sheer number of permutations of EAE models makes their use a minefield for experimental error, and diving into the literature is often not enough to develop the needed level of expertise to use EAE most effectively, Becher says. Leptin is a hormonal peptide produced by adipocytes that participates in the regulation of energy balance and food intake in the organism [32].


This immunisation leads to multiple small disseminated lesions of demyelination as well as micro-necroses in the brain and spinal cord and the onset of clinical symptoms. Views Read Edit View history.

EAE was motivated by observations during the convalescence from viral diseases by Thomas M. From Wikipedia, the free encyclopedia. In the past 20 years, researchers have used transgenic techniques to develop new versions of the model and to expand its utility. Please sign in or create an account.

Experimental autoimmune encephalomyelitis – Wikipedia

Sobre la base de nuestra experiencia, hay varios rEasons de este. Relative auttoinmune was performed using the following formula [18]: This storage period was necessary in order to prevent the death of the animals associated with the use of freshly prepared PT. Get cutting-edge science videos from J o VE sent straight to your inbox every month. La figura ha sido modificado a partir de Barthelmes et al.

Experimental Autoimmune Encephalomyelitis

Immunological update on multiple sclerosis. They were used after a laboratory adaptation period of one week. You must be signed in to post a comment. The most commonly used antigens in rodents are spinal cord homogenate SCHpurified myelin, myelin protein such as MBPPLPand MOGor peptides of these proteins, all ebcefalomielitis in distinct models with different disease characteristics regarding both immunology and pathology.

IL drives a pathogenic T cell population that induces autoimmune inflammation.

EAE generally targets the spinal cord and sometimes the cerebellum, causing inflammation followed by demyelination and axonal damage. The immunological basis for treatment of multiple sclerosis. Publication guidelines for refereeing and reporting on animal use in experimental autoimmune encephalomyelitis. Statistical analysis Statistical analyses were performed using the Prism 5.

Model evaluation Clinical monitoring of EAE The clinical severity of the disorder in all three experimental groups was monitored by daily double-blind observations, performed always at the same hour, by a trained observer, starting from day 0 and until day 28 after induction.