ISO 20776-1 PDF

ISO 20776-1 PDF

ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of.

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ISO documents of relevance to antimicrobial susceptibility testing These documents are available for purchase from ISO or national standards organisations. We use cookies to make our website easier to use and to better understand your needs. Considering only data for dilution series prepared in Spain, no systematic difference was detected between ISO dilution and serial dilution. 20776–1 Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – Part 1: Susceptibility testing of pathogenic fungi with itraconazole: Essais de receptivite d’agents infectieux et evaluation des performances des dispositifs de receptivite antimicrobienne.

When there are no breakpoints? An optimized analytical method of fluconazole in human plasma by high-performance liquid chromatography with ultraviolet detection and its application to a bioequivalence study.

Steering Committee to Presentations and statistics It is generally accepted that broth MIC tests are reproducible to within one doubling dilution of the real end point i.

Click to learn more. Development and validation of a high-performance liquid chromatography assay for posaconazole utilizing solid-phase extraction. Rationale documents for antifungals Single-step extraction of fluconazole from ixo by ultra-filtration for the measurement of its free concentration by high performance liquid chromatography.

BS EN ISO 20776-1:2006

ITC precipitation after thaw-freeze cycles during transport is the most probable explanation. National Center for Biotechnology InformationU. Selective high-performance liquid chromatographic assay for itraconazole and hydroxyitraconazole in plasma from human immunodeficiency virus-infected patients. Author information Article notes Copyright and License information Disclaimer.

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Trays were done in duplicate on separate days at each center and then shipped frozen by special delivery to Spain for further chromatographic concentration determinations. Projects and data submission While this is the gold standard for preparation of dilution series, the question has risen if modern pipettes are sufficiently precise to allow dilution series to be prepared as serial dilutions without a loss of accuracy.

Previous versions of documents Veterinary Susceptibility Testing Notably, although relative errors were greater for the lowest concentrations, we did not observe an increasing inaccuracy when performing serial dilutions compared to dilutions performed following the ISO procedure as would have been expected if the precision of modern pipettes had been insufficient.

EUCAST: External documents

Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility testing devices. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices Evaluation of performance of antimicrobial susceptibility test devices BS EN FLC eluted at 4. In order to determine and compare the accuracy of each method described for microdilution, two consecutive HPLC determinations of the azole concentration were calculated from different wells of trays prepared according ISO recommendations, serial dilutions for hydrophobic drugs, and serial dilutions for hydrophilic compounds.

In summary, our data suggest that microdilution trays for hydrophilic drugs such as FLC can be accurately prepared following the ISO recommendations or the serial 2-fold dilution schemes. These modifications are included in a separate table. Our data showed similar precision and accuracy within each laboratory of the three procedures used for FLC microplate preparation. Careful control and standardisation is required for intra- and inter-laboratory reproducibility, as results may be significantly influenced by the method used.

Broth dilution is a technique in which containers holding identical volumes of broth with antimicrobial agent solutions in incrementally usually geometrically increasing concentrations are inoculated with a known number of microorganisms. Clinical breakpoints for antimycobacterial agents This website is best viewed with browser version of up to Microsoft Internet Explorer 8 or Firefox 3. ITC is a weakly basic drug pKa ca. MIC methods are used in resistance surveillance, comparative testing of new agents, to establish the susceptibility of organisms that give equivocal results in routine tests, for tests on organisms where routine tests may be unreliable and when a quantitative result is required for clinical management.

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Rapid AST in bloodcultures Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. International Organization for Standards, Geneva, Switzerland.

However, itraconazole trays showed a significant lack of reproducibility that was independent of which method was followed. A multicenter study was conducted to assess the accuracy of the ISO standard and the serial 2-fold dilution procedures for antifungal susceptibility testing.

This article has been cited by other articles in PMC. Published online Mar HPLC is a sensitive and reproducible method for determining levels of azole components in a great variety of matrices, as has been confirmed in previous reports 12510 – 121519 – 21 Quality control procedures of AST should be implemented 2077-1 order to minimize the inaccuracy of dilution methods and lack 200776-1 reproducibility when hydrophobic compounds such as ITC are tested.

Relevant external documents

Example of a quick liquid chromatographic assay of itraconazole in serum. Acknowledgments We have no conflict of interest to declare. Susceptibility testing of yeasts Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.